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KMID : 1134820100390101433
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 10 p.1433 ~ p.1438
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The Antioxidative and Antimicrobial Effects of Internal Organs of Aplysia kurodai Fractions
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Shin Mi-Ok
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Abstract
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This study was carried out to investigate the antioxidative and antimicrobial activities of internal organs of Aplysia kurodai (AK). The internal organs of AK were extracted with methanol (AKM), which was then further fractionated into four subfractions by using solvent partition method, affording hexane (AKMH), methanol (AKMM), butanol (AKMB), and aqueous (AKMA) soluble fractions. The antioxidative activity of fractions from AK was investigated by measuring the scavenging activities of AK against DPPH radical, peroxynitrite (ONOO-) and reactive oxygen species (ROS). Among the various solvent fractions, AKMB showed a marked scavenging effect against DPPH radical, peroxynitrite (ONOO-) and reactive oxygen species (ROS). The antimicrobial activity was increased in proportion to its concentration by the paper disk method. Among the various solvent fractions, AKMM fractions of AK showed the strongest antimicrobial activities. The methanol extracts exhibited antimicrobial activity against all organisms tested, while the hexane extracts showed antimicrobial activity only against Proteus vulgaris. The results suggest that the AK may be suitable for development as a food preservative and alternative antioxidant.
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KEYWORD
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antioxidative activity, antimicrobial activity, internal organs of Aplysia kurodai (AK)
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